Resources for sharing

Expression vector maps for biosensors

For detailed usage of these biosensors in imaging, or for plasmid requests, please contact Dr. Kui Wang.

IDVectorDescriptionReference
K2pcDNA-GRITTryptophan sensor expressed in mammalian cellCell Disc, 2023
K3pcDNA-GRITolTryptophan control sensor expressed in mammalian cellCell Disc, 2023
K4pcDNA-mito-GRITMitochondrial Tryptophan sensor expressed in mammalian cellBMC Biol, 2024
K5pcDNA-mito-GRITolMitochondrial Tryptophan control sensor expressed in mammalian cellBMC Biol, 2024
K6✨GEMCPPU1.0 SensorRatiometric biosensor for detecting Forchlorfenuron (CPPU)Manuscript in preparation
K7✨GEMTrpA1.0 SensorNovel sensor for monitoring Tryptamine dynamics in vivoUnder revision

Ratio Imaging Analyzer (RIA)

Ratio Imaging Analyzer (RIA) is a lightweight, user-friendly tool designed for processing dual-channel ratiometric fluorescence data (e.g., Calcium, FRET, and metabolic sensors). It features automated ratiometric heatmap generation, smart background subtraction, and real-time ROI plotting.
PyPI Download Latest Release Python Version License DOI Developer GitHub stars Visitors
Automated Workflow
Auto-alignment & ratiometric heatmap generation
Interactive Analysis
Real-time ROI monitoring & curve plotting

🖥️ Other Ready-to-Use Lab Software


1. mProcess
A self-developed tool for microplate data preprocessing.
GitHub RepoDownloadLicenseDeveloperOpen SourceDOI
mProcess
Batch data processing interface.
2. zGrating
A self-developed visual stimulus generator.
DownloadLicenseDeveloper
zGrating
Radial grating pattern generation
3. zStimuli
Controls Arduino via serial communication for multimodal stimuli
DownloadLicenseDeveloper
zStimuli
Control panel for experimental stimuli

Protocols in our hands

Useful codes

bundle exec jekyll serve --livereload
jekyll clean