Resources for sharing
Expression vector maps for biosensors
For detailed usage of these biosensors in imaging, or for plasmid related to any other amino acid metabolites requests, please contact Dr. Kui Wang.
| ID | Vector | Description | Reference |
|---|---|---|---|
| K2 | pcDNA-GRIT | Tryptophan sensor expressed in mammalian cell | Cell Disc, 2023 |
| K3 | pcDNA-GRITol | Tryptophan control sensor expressed in mammalian cell | Cell Disc, 2023 |
| K4 | pcDNA-mito-GRIT | Mitochondrial Tryptophan sensor expressed in mammalian cell | BMC Biol, 2024 |
| K5 | pcDNA-mito-GRITol | Mitochondrial Tryptophan control sensor expressed in mammalian cell | BMC Biol, 2024 |
| K6 | ✨GEMCPPU1.0 Sensor | Ratiometric biosensor for detecting Forchlorfenuron (CPPU) | Manuscript in preparation |
| K7 | ✨GEMTrpA1.0 Sensor | Novel sensor for monitoring Tryptamine dynamics in vivo | Under revision |
| K8 | ✨GEMAdo1.0 Sensor | An ultrasensitive, high-contrast sensor for monitoring Adenosine dynamics in vivo. We welcome collaboration to test in diverse model organisms. | Available for beta testing |
Ratio Imaging Analyzer (RIA)
Ratio Imaging Analyzer (RIA) is a lightweight, user-friendly tool designed for processing dual-channel ratiometric fluorescence data (e.g., Calcium, FRET, and metabolic sensors). It features automated ratiometric heatmap generation, smart background subtraction, and real-time ROI plotting.
Automated Workflow
Auto-alignment & ratiometric heatmap generationInteractive Analysis
Real-time ROI monitoring & curve plotting🖥️ Other Ready-to-Use Lab Software
1. mProcess
A self-developed tool for microplate data preprocessing.
2. zGrating
A self-developed visual stimulus generator.
3. zStimuli
Controls Arduino via serial communication for multimodal stimuli
Protocols in our hands
- High-throughput recombinant DNA preparation
- In vitro transcription for mRNA preparation
- Biosensor protein purification
- Fluorescent biosensor screening and modification
Useful codes
bundle exec jekyll serve --livereload jekyll clean