Resources for sharing

Expression vector maps for biosensors

For detailed usage of these biosensors in imaging, or for plasmid related to any other amino acid metabolites requests, please contact Dr. Kui Wang.

IDVectorDescriptionReference
K2pcDNA-GRITTryptophan sensor expressed in mammalian cellCell Disc, 2023
K3pcDNA-GRITolTryptophan control sensor expressed in mammalian cellCell Disc, 2023
K4pcDNA-mito-GRITMitochondrial Tryptophan sensor expressed in mammalian cellBMC Biol, 2024
K5pcDNA-mito-GRITolMitochondrial Tryptophan control sensor expressed in mammalian cellBMC Biol, 2024
K6✨GEMCPPU1.0 SensorRatiometric biosensor for detecting Forchlorfenuron (CPPU)Manuscript in preparation
K7✨GEMTrpA1.0 SensorNovel sensor for monitoring Tryptamine dynamics in vivoUnder revision
K8✨GEMAdo1.0 Sensor An ultrasensitive, high-contrast sensor for monitoring Adenosine dynamics in vivo.
We welcome collaboration to test in diverse model organisms.		 Available for beta testing
More sensors for metabolites are coming...



Ratio Imaging Analyzer (RIA)

Ratio Imaging Analyzer (RIA) is a lightweight, user-friendly tool designed for processing dual-channel ratiometric fluorescence data (e.g., Calcium, FRET, and metabolic sensors). It features automated ratiometric heatmap generation, smart background subtraction, and real-time ROI plotting.
PyPI Download Latest Release Python Version License DOI Developer GitHub stars
Automated Workflow
Auto-alignment & ratiometric heatmap generation
Interactive Analysis
Real-time ROI monitoring & curve plotting

🖥️ Other Ready-to-Use Lab Software


1. mProcess
A self-developed tool for microplate data preprocessing.
GitHub RepoDownloadLicenseDeveloperOpen SourceDOI
mProcess
Batch data processing interface.
2. zGrating
A self-developed visual stimulus generator.
DownloadLicenseDeveloper
zGrating
Radial grating pattern generation
3. zStimuli
Controls Arduino via serial communication for multimodal stimuli
DownloadLicenseDeveloper
zStimuli
Control panel for experimental stimuli

Fiji ImageJ Plugin

Biosensor Tools is a suite of open-source ImageJ/Fiji plugins designed for ratiometric imaging, motion correction, metadata inspection, and AI-powered denoising. As active developers of ImageJ plugins, our team is dedicated to providing biologists with simple and handy analysis tools to facilitate the study of neurochemical dynamics in live animals using genetically encoded fluorescent biosensors.
📥 Quick Installation via Fiji
  1. Open Fiji and navigate to Help > Update...
  2. Click Manage update sites, then click Add update site.
  3. Enter Name: Biosensor Tools and URL: https://sites.imagej.net/Biosensor-Tools/
  4. Close the window and click Apply changes.

RIA-J (Ratio Imaging Analyzer)
Publication-grade ratiometric analysis. Features background subtraction, pixel-by-pixel ratio calculation, and IMD display mode.
GitHub Repo ImageJ Wiki
RIA-J preview
FIA (Fluorescence Image Aligner)
Motion correction & Registration. Robustly stabilizes live-animal time-lapse datasets (Global & Dense Flow) before quantification.
GitHub Repo ImageJ Wiki
FIA preview
MIA (Metadata Inspection Analyzer)
Metadata explorer. Instantly extracts hidden tags (Olympus .oir, OME-XML) and exports summaries to Excel/CSV.
GitHub Repo ImageJ Wiki
MIA preview
NIA (Neural Inference Assistant)
Zero-setup AI denoising. Runs deep learning models (DnCNN) directly in Java via ONNX Runtime. No Python/CUDA required. Supports 5D hyperstacks.
GitHub Repo ImageJ Wiki
NIA preview
WinMan (Window Manager)
Workspace accelerator. Efficiently manages, tiles, and filters multiple image windows to keep your ImageJ workspace clean.
GitHub Repo ImageJ Wiki
WinMan preview

Protocols in our hands

Useful codes

bundle exec jekyll serve --livereload
jekyll clean